Rabbit anti-eIF4AIII/EIF4A3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-980A-T maps to a region between residue 1 and 50 of human Eukaryotic Translation Initiation Factor 4A3 using the numbering given in entry NP_055555.1 (GeneID 9775).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF4AIII/EIF4A3 immobilized on solid support.
The epitope recognized by A302-980A maps to a region between residue 1 and 50 of human Eukaryotic Translation Initiation Factor 4A3 using the numbering given in entry NP_055555.1 (GeneID 9775).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Eukaryotic initiation factor 4A-III is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. eIF4AIII is an ATP-dependent helicase and a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs.
Alternate Names
ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DDX48; DEAD (Asp-Glu-Ala-Asp) box polypeptide 48; DEAD box protein 48; eIF4AIII; eIF-4A-III; eIF4A-III; eukaryotic initiation factor 4A-III; eukaryotic initiation factor 4A-like NUK-34; eukaryotic translation initiation factor 4A; Eukaryotic translation initiation factor 4A isoform 3; Fal1; hNMP 265; MUK34; NMP 265; NMP265; nuclear matrix protein 265; NUK34; RCPS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.