Rabbit anti-eIF4B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-767A maps to a region between residue 561 and 611 of human eukaryotic translation initiation factor 4B using the numbering given in entry NP_001408.2 (GeneID 1975).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to eIF4B immobilized on solid support.
The epitope recognized by A301-767A-T maps to a region between residue 561 and 611 of human eukaryotic translation initiation factor 4B using the numbering given in entry NP_001408.2 (GeneID 1975).
Additional Product Information
Eukaryotic initiation factor 4B (eIF4B) is a factor required for the binding of mRNA to ribosomes for the initiation of translation. eIF4B has been demonstrated to modulate the helicase activity of eIF4A, a component of the eIF4F complex.
Alternate Names
EIF-4B; eukaryotic translation initiation factor 4B; PRO1843
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.