Rabbit anti-EPRS Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A303-959A maps to a region between residue 950 to 1000 of human Glutamyl-prolyl-tRNA Synthetase using the numbering given in entry NP_004437.2 (GeneID 2058).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EPRS immobilized on solid support.
The epitope recognized by A303-959A-T maps to a region between residue 950 to 1000 of human Glutamyl-prolyl-tRNA Synthetase using the numbering given in entry NP_004437.2 (GeneID 2058).
Additional Product Information
EPRS (glutamyl-prolyl-tRNA synthetase) catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P07814].
Alternate Names
bifunctional aminoacyl-tRNA synthetase; bifunctional glutamate/proline--tRNA ligase; cell proliferation-inducing gene 32 protein; EARS; GLUPRORS; GluRS; glutamate tRNA ligase; Glutamate--tRNA ligase; glutamatyl-prolyl-tRNA synthetase; glutaminyl-tRNA synthetase; Glutamyl-tRNA synthetase; HLD15; PARS; PIG32; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline-tRNA ligase; Proline--tRNA ligase; prolyl-tRNA synthetase; QARS; QPRS
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.