Rabbit anti-EVI5 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
The EVI5 oncogene has been identified as a protein that is localized to the centrosome and mitotic spindle, and in association with the aurora B complex, facilitates cytokinesis. EVI5 also appears to prevent premature APC/C activation and mitotic progression by stabilizing the anaphase-promoting complex/cyclosome (APC/C) inhibitor, Emi1, as well as cyclin A. EVI5 has also been demonstrated to be a binding partner of the Rab11 GTPase that regulates intracellular transport and cytokinesis.
Alternate Names
dJ846D11.1 (ecotropic viral integration site 5); ecotropic viral integration site 5 protein homolog; EVI-5; NB4S; neuroblastoma stage 4S gene protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.