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Rabbit anti-EWS Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
EWS
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 125 and 175
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to EWS immobilized on solid support.

The epitope recognized by A300-417A-T maps to a region between residues 100 and 150 of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to EWS immobilized on solid support.

The epitope recognized by A300-417A maps to a region between residues 100 and 150 of human Ewing sarcoma breakpoint region 1 using the numbering given in SwissProt entry Q01844 (GeneID 2130).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

EWS is a member of a family of RNA-binding proteins that may serve as adapters that bridge RNA polymerase II complex and RNA splicing factors. Translocations and fusions between EWS and the ETS transcription factors promote oncogenesis and are associated with Ewing’s family tumors.

2130
EWSR1
EWS RNA binding protein 1
Q01844
RNA-binding protein EWS

Alternate Names

bK984G1.4; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS-Fli1 (type 1) oncogene; EWS; EWS oncogene; EWS RNA-binding protein variant 6; EWS-FLI1; RNA-binding protein EWS

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.