Rabbit anti-Exonuclease 1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-640A maps to a region between residue 796 and 846 of human exonuclease 1 using the numbering given in entry NP_006018.3 (GeneID 9156).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Exonuclease 1 immobilized on solid support.
The epitope recognized by A302-640A-T maps to a region between residue 796 and 846 of human exonuclease 1 using the numbering given in entry NP_006018.3 (GeneID 9156).
Additional Product Information
Exonuclease 1 is a 5'-3' double-stranded DNA exonuclease which may also possess a cryptic 3'-5' double-stranded DNA exonuclease activity. Exonuclease 1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. It also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Exonuclease 1 is required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes and is essential for male and female meiosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UQ84].
Alternate Names
exonuclease 1; Exonuclease I; HEX1; hExo1; hExoI; rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.