Rabbit anti-EXOSC10 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Exosome component 10 (EXOSC10) is a putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRN. It is also involved in the elimination of RNA-processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q01780].
Alternate Names
Autoantigen PM/Scl 2; autoantigen PM-SCL; exosome component 10; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; p2; p3; p4; PM/Scl-100; PMSCL; PM-Scl; PMSCL2; polymyositis/scleroderma autoantigen 100 kDa; polymyositis/scleroderma autoantigen 2; RRP6; Rrp6p
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.