Rabbit anti-EYA3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-689A maps to a region between residue 1 and 30 of human eyes absent homolog 3 using the numbering given in entry NP_001981.2 (GeneID 2140).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EYA3 immobilized on solid support.
The epitope recognized by A302-689A-T maps to a region between residue 1 and 30 of human eyes absent homolog 3 using the numbering given in entry NP_001981.2 (GeneID 2140).
Additional Product Information
EYA3 (eyes absent homolog 3) functions as a tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). EYA3 promotes efficient DNA repair by dephosphorylating H2AX and promoting the recruitment of DNA repair complexes containing MDC1. EYA3 coactivates SIX1. Together with SIX1 and DACH2 EYA3 seems to be involved in myogenesis. EYA3 may be involved in development of the eye [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q99504].
Alternate Names
eyes absent 3; eyes absent homolog 3
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.