Rabbit anti-EZH2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-197A-T maps to a region between residue 696 to 746 of human Enhancer of Zeste Homolog 2 using the numbering given in entry CAA64955.1 (GeneID 2146).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to EZH2 immobilized on solid support.
The epitope recognized by A304-197A maps to a region between residue 696 to 746 of human Enhancer of Zeste Homolog 2 using the numbering given in entry CAA64955.1 (GeneID 2146).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Enhancer of zeste homolog 2 (EZH2) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems [taken from NCBI Entrez Gene (Gene ID: 2146)].
Alternate Names
enhancer of zeste homolog 2; ENX1; ENX-1; EZH2b; histone-lysine N-methyltransferase EZH2; KMT6; KMT6A; lysine N-methyltransferase 6; WVS; WVS2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.