Rabbit anti-FAM96B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-835A maps to a region between residue 1 and 50 of human Mitotic spindle-associated MMXD complex subunit MIP18 using the numbering given in entry Q9Y3D0.1 (GeneID 51647).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FAM96B immobilized on solid support.
The epitope recognized by A305-835A-T maps to a region between residue 1 and 50 of human Mitotic spindle-associated MMXD complex subunit MIP18 using the numbering given in entry Q9Y3D0.1 (GeneID 51647).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
FAM96B is a component of the cytosolic iron-sulfur protein assembly (CIA) complex, a multiprotein complex that mediates the incorporation of iron-sulfur cluster into extramitochondrial Fe/S proteins. As a CIA complex component and in collaboration with CIAO1 and MMS19, FAM96B binds to and facilitates the assembly of most cytosolic-nuclear Fe/S proteins. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation, probably by facilitating iron-sulfur cluster assembly into ERCC2/XPD. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y3D0].
Alternate Names
CGI-128; CIA2B; cytosolic iron-sulfur assembly component 2B; FAM96B; family with sequence similarity 96 member B; MIP18; mitotic spindle-associated MMXD complex subunit MIP18; MSS19-interacting protein of 18 kDa; Protein FAM96B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.