Rabbit anti-FBXO11 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-177A maps to a region between residue 1 and 50 of human F-box only protein 11 using the numbering given in entry NP_001177203.1 (GeneID 80204).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FBXO11 immobilized on solid support.
The epitope recognized by A301-177A-T maps to a region between residue 1 and 50 of human F-box only protein 11 using the numbering given in entry NP_001177203.1 (GeneID 80204).
Additional Product Information
FBXO11 is a member of the F-box protein family and component of the Skp1-Cullin1-F-box (SCF) ubiquitin ligase complex. FBXO11 has been shown to interact with p53 and suppress its activity. Suppression of p53 activity is achieved via Nedd8 conjugation to p53.
Alternate Names
F-box only protein 11; FBX11; IDDFBA; PRMT9; protein arginine N-methyltransferase 9; ubiquitin protein ligase E3 component n-recognin 6; UBR6; UG063H01; VIT1; VIT-1; vitiligo-associated protein 1; vitiligo-associated protein VIT-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.