Rabbit anti-FH/Fumarate Hydratase Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-262A maps to a region between residue 150 to 200 of human Fumarate hydratase, mitochondrial using the numbering given in entry P07954.3 (GeneID 2271).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FH/Fumarate Hydratase immobilized on solid support.
The epitope recognized by A305-262A-T maps to a region between residue 150 to 200 of human Fumarate hydratase, mitochondrial using the numbering given in entry P07954.3 (GeneID 2271).
Additional Product Information
Fumarate is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used [taken from NCBI Entrez Gene (Gene ID: 2271)].
Alternate Names
epididymis secretory sperm binding protein; FMRD; fumarase; fumarate hydratase, mitochondrial; HLRCC; LRCC; MCL; MCUL1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.