Rabbit anti-FOG1/ZFPM1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-431A maps to a region between residue 175 and 225 of human friend of GATA-1 (zinc finger protein, multitype 1) using the numbering given in entry NP_722520.2 (GeneID 161882).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to FOG1/ZFPM1 immobilized on solid support.
The epitope recognized by A301-431A-T maps to a region between residue 175 and 225 of human friend of GATA-1 (zinc finger protein, multitype 1) using the numbering given in entry NP_722520.2 (GeneID 161882).
Additional Product Information
FOG1/ZFPM1 was identified in a yeast two-hybrid screen for GATA-1 interacting proteins. GATA-1 is zinc finger transcription factor whose expression is restricted to hematopoietic cells and plays and essential role in erythroid development. FOG1/ZFPM1 is highly expressed in erythroid cells and megakaryocytes and appears to play both a GATA-1-dependent and -independent role in erythropoiesis and megakaryopoiesis. FOG1/ZFPM1 associates with GATA-1 as well as GATA-2 and GATA-3 as a heterodimer to activate or repress transcription.
Alternate Names
FOG; FOG1; FOG-1; friend of GATA 1; friend of GATA protein 1; ZC2HC11A; zinc finger protein 89A; Zinc finger protein multitype 1; zinc finger protein ZFPM1; zinc finger protein, multitype 1; ZNF408; ZNF89A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.