Rabbit anti-FOXO3a Recombinant Monoclonal Antibody [BLR140H]
Product Details
Specifications
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
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Immunogen was a peptide representing a region between residue 450 and 500 of human Forkhead box protein O3 using the numbering given in entry NP_001446.1 (Gene ID 2309).
Additional Product Information
Forkhead box protein 03A (FOXO3a) belongs to a large family of Forkhead proteins that possesses a winged-helix DNA-binding domain called Forkhead box (FOX). FOXO3a is a transcription factor that functions to induce apoptosis in the absence of survival factors and in response to oxidative stress. Under conditions of oxidative stress FOXO3a is acetylated and localized to the nucleus where it may act as a transcriptional repressor.
Alternate Names
AF6q21; AF6q21 protein; FKHRL1; FKHRL1P2; forkhead box O3A; forkhead box protein O3; forkhead homolog (rhabdomyosarcoma) like 1; forkhead in rhabdomyosarcoma-like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1; FOXO2; FOXO3A
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.