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Rabbit anti-GAB1 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
GAB1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 644 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to GAB1 immobilized on solid support.

The epitope recognized by A303-288A maps to a region between residue 644 and 694 of human GRB2-Associated Binding Protein 1 using the numbering given in entry NP_002030.2 (GeneID 2549).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to GAB1 immobilized on solid support.

The epitope recognized by A303-288A-T maps to a region between residue 644 and 694 of human GRB2-Associated Binding Protein 1 using the numbering given in entry NP_002030.2 (GeneID 2549).
Country of Origin
USA

Additional Product Information

GRB2-associated-binding protein 1 (GAB1) is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis [taken from NCBI Entrez Gene (Gene ID: 2549)].

2549
GAB1
GRB2 associated binding protein 1
Q13480
GRB2-associated-binding protein 1

Alternate Names

DFNB26; GRB2-associated binder 1; GRB2-associated-binding protein 1; growth factor receptor bound protein 2-associated protein 1

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.