Rabbit anti-Gemin5 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-325A maps to a region between residue 1360 and 1410 of human gem (nuclear organelle) associated protein 5 using the numbering given in entry NP_056280.1 (GeneID 25929).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Gemin5 immobilized on solid support.
The epitope recognized by A301-325A-T maps to a region between residue 1360 and 1410 of human gem (nuclear organelle) associated protein 5 using the numbering given in entry NP_056280.1 (GeneID 25929).
Additional Product Information
Gemin5 is a component of the SMN (survival of motor neurons) complex. The SMN complex is composed of the SMN protein and several Gemins (2-7) and is involved in the assembly of snRNPs in the cytoplasm and their transport to the nucleus. The SMN complex localizes to nuclear focal structures called gems or Gemini of coiled bodies. Gemin 5 has been shown to be responsible for recognizing and binding the snRNP code during snRNP biogenesis. Recent studies have shown that Gemin 5 is rarely detectable in SMN complexes associated with gems/Cajal bodies in the nucleus and may play a specific role in cytoplasmic SMN complexes. It has also been shown to function as a scaffold protein for ASK1-JNK1 signaling.
Alternate Names
gem-associated protein 5; Gemin5; GEMIN-5
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.