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Rabbit anti-GGCT Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
GGCT
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 138 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to GGCT immobilized on solid support.

The epitope recognized by A305-563A maps to a region between residue 138 to 188 of human Gamma-glutamylcyclotransferase using the numbering given in entry O75223.1 (GeneID 79017).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to GGCT immobilized on solid support.

The epitope recognized by A305-563A-T maps to a region between residue 138 to 188 of human Gamma-glutamylcyclotransferase using the numbering given in entry O75223.1 (GeneID 79017).

Country of Origin
USA

Additional Product Information

Gamma-glutamylcyclotransferase (GGCT) catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. GGCT may also play a role in cell proliferation, and the expression of GGCT is a potential marker for cancer [taken from NCBI Entrez Gene (Gene ID: 79017)].

79017
GGCT
gamma-glutamylcyclotransferase
O75223
Gamma-glutamylcyclotransferase

Alternate Names

C7orf24; CRF21; cytochrome c-releasing factor 21; gamma -glutamyl cyclotransferase; gamma-glutamylcyclotransferase; GCTG; GGC

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.