Rabbit anti-GIT1 IHC Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by IHC-00527 maps to a region between residue 375 and 425 of human G protein-coupled receptor kinase interacting ArfGAP 1 using the numbering given in entry NP_054749.2 (GeneID 28964).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to GIT1 immobilized on solid support.
The epitope recognized by IHC-00527-T maps to a region between residue 375 and 425 of human G protein-coupled receptor kinase interacting ArfGAP 1 using the numbering given in entry NP_054749.2 (GeneID 28964).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
G-protein-coupled receptor kinase interactor 1 (GIT1) and G-protein-coupled receptor kinase interactor 2 (GIT2) are GTPase-activating proteins for ADP-ribosylation (Arf) GTPases. GIT1 and GIT2 regulate Arf activity and the sequestration of activated G protein-coupled receptors from the surface of the cell. These activities have effects on multiple cellular processes such as cell migration, T-cell activation, and centrosome dynamics. Both GIT proteins also serve as scaffolds to link signaling molecules.
Alternate Names
ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT1; CAT-1; cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.