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Rabbit anti-Glu-Glu Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog # A190-111A
Target:
Glu-Glu Tag
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.1 mg)

A190-111A
$268.00
Qty:

Product Details

Specifications

Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
Rabbits were immunized with a synthetic peptide representing amino acid residues 314 - 319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The Glu-Glu tag is an epitope tag with a 6-residue sequence of EYMPME. Derived from the middle T antigen of mouse polyomavirus, Glu-Glu tags can help with detection of proteins in western blot, IP, or microscopic techniques.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.