Rabbit anti-Glu-Glu Tag Antibody Affinity Purified
Product Details
Specifications
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The Glu-Glu tag is an epitope tag with a 6-residue sequence of EYMPME. Derived from the middle T antigen of mouse polyomavirus, Glu-Glu tags can help with detection of proteins in western blot, IP, or microscopic techniques.
Applications
Not all listed applications have been specifically tested by our laboratory.
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.