Rabbit anti-GNL3 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-599A-T maps to a region between residues 1 and 50 of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354). Antibody was affinity purified using an epitope specific to GNL3 immobilized on solid support.
The epitope recognized by A300-599A maps to a region between residues 1 and 50 of human Guanine Nucleotide Binding Protein-Like 3 (Nucleostemin) using the numbering given in entry NP_055181.3 (GeneID 26354).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
GNL3 (guanine nucleotide binding protein-like 3), also known as nucleostemin (NS), was identified as a protein highly expressed in stem cells and downregulated in differentiated cells.GNL3 has also been found to be highly expressed in some cancer cell lines, and may play an important role in the control of cell proliferation. GNL3 functions as a GTPase and functions in the processing of nucleolar pre-rRNA.
Alternate Names
C77032; E2IG3; E2-induced gene 3 protein; estradiol-induced nucleotide binding protein; guanine nucleotide binding protein-like 3 (nucleolar); guanine nucleotide-binding protein-like 3; NNP47; novel nucleolar protein 47; NS; nucleolar GTP-binding protein 3; nucleostemin
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.