Rabbit anti-GPI Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Glucose-6-phosphate isomerase (GPI) is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. GPI functions in different capacities inside and outside the cell. In the cytoplasm, GPI is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in the GPI gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment [taken from NCBI Entrez Gene (Gene ID: 2821)].
Alternate Names
AMF; autocrine motility factor; glucose-6-phosphate isomerase; GNPI; GPI; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; NLK; oxoisomerase; PGI; PHI; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; SA36; SA-36; Sperm antigen 36; sperm antigen-36
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.