Rabbit anti-GRAF Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-340A maps to a region between residue 611 to 661 of human GTPase Regulator Associated with Focal Adhesion Kinase pp125(FAK) using the numbering given in entry NP_055886.1 (GeneID 23092).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to GRAF immobilized on solid support.
The epitope recognized by A304-340A-T maps to a region between residue 611 to 661 of human GTPase Regulator Associated with Focal Adhesion Kinase pp125(FAK) using the numbering given in entry NP_055886.1 (GeneID 23092).
Additional Product Information
Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. GTPase regulator associated with focal adhesion kinase (GRAF) is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc4 [taken from NCBI Entrez Gene (Gene ID: 23092)].
Alternate Names
GRAF; GRAF1; GTPase regulator associated with focal adhesion kinase; GTPase regulator associated with focal adhesion kinase pp125(FAK); oligophrenin-1-like protein; OPHN1L; OPHN1L1; rho GTPase-activating protein 26; Rho-type GTPase-activating protein 26
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.