Rabbit anti-Hakai Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-969A maps to a region between residue 441 and 491 of human E3 ubiquitin-protein ligase Hakai using the numbering given in entry NP_079090.2 (GeneID 79872).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Hakai immobilized on solid support.
The epitope recognized by A302-969A-T maps to a region between residue 441 and 491 of human E3 ubiquitin-protein ligase Hakai using the numbering given in entry NP_079090.2 (GeneID 79872).
Additional Product Information
Hakai is an E3-ubiquitin ligase and an E-cadherin binding protein that plays a role in modulating cell adhesion.
Alternate Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence-like 1; casitas B-lineage lymphoma-transforming sequence-like protein 1; Cbl proto-oncogene like 1, E3 ubiquitin protein ligase; Cbl proto-oncogene, E3 ubiquitin protein ligase-like 1; c-Cbl-like protein 1; E3 ubiquitin-protein ligase Hakai; E-cadherin binding protein E7; HAKAI; RING finger protein 188; RING-type E3 ubiquitin transferase Hakai; RNF188
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.