Rabbit anti-HCF1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-400A maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HCF1 immobilized on solid support.
The epitope recognized by A301-400A-T maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
Additional Product Information
HCF1 was originally identified as a host-cell factor utilized by the herpes simplex virus to promote viral transcription. In the cell, HCF1 functions as a transcriptional co-regulator involved in the modification of histones and cell cycle progression.
Alternate Names
C1 factor; CFF; HCF; HCF1; HCF-1; HFC1; host cell factor 1; MRX3; PPP1R89; protein phosphatase 1, regulatory subunit 89; VCAF; VP16 accessory protein; VP16-accessory protein
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.