Rabbit anti-HDAC2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-705A maps to a region between residue 425 and the C-terminus (residue 488) of human Histone Deacetylase 2 using the numbering given in entry NP_001518.1 (GeneID 3066).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HDAC2 immobilized on solid support.
The epitope recognized by A300-705A-T maps to a region between residue 425 and the C-terminus (residue 488) of human Histone Deacetylase 2 using the numbering given in entry NP_001518.1 (GeneID 3066).
Additional Product Information
Histone deacetylase 2 (HDAC2) is a class I histone deacetylase that catalyzes the removal of the acetyl group on lysine residues of the N-terminus of the core histones H2A, H2B, H3, and H4. HDAC2 is a component of multiple deacetylating complexes such as Sin3, NuRD, and CoRest that function to repress gene transcription.
Alternate Names
HD2; histone deacetylase 2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.