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Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
HIF2-alpha
Reactivity:
Human
Host:
Rabbit
Clonality:
Recombinant Monoclonal
Clone:
BL-95-1A2
Format:
Whole IgG
Immunogen:
Between 400 and 450
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Purified
100 µl (10 blots)

20 µl (2 blots)

100 µg (BSA-Free)

$127.00 $794.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
400 µg/ml 1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Request Formulation Change Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Request Formulation Change
Production Procedures
Recombinant antibody was purified from cell culture supernatant.

The epitope recognized by A700-003-T maps to a region between residues 400 and 450 of human endothelial PAS domain protein 1 using the numbering given in entry NP_001421.2 (GeneID 2034).
Recombinant antibody was purified from cell culture supernatant.

The epitope recognized by A700-003 maps to a region between residues 400 and 450 of human endothelial PAS domain protein 1 using the numbering given in entry NP_001421.2 (GeneID 2034).
Country of Origin
USA

Additional Product Information

The gene EPAS1 encodes for the transcription factor endothelial PAS domain-containing protein 1, and is induced as oxygen levels fall. Commonly called hypoxia inducible factor 2A (HIF2a), this protein is involved in the induction of oxygen regulated genes, binding to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. An important cancer biomarker, HIF2a may also play a role in Tibetan high-altitude adaptation (Am J Hum Genet. 2015 Jul 2; 97(1): 54–66.) and mutations in the EPAS1 gene are associated with erythrocytosis familial type 4 [taken from NCBI Entrez Gene (Gene ID: 2034) and UniProtKB (Entry: Q99814)].

2034
EPAS1
endothelial PAS domain protein 1
Q99814
Endothelial PAS domain-containing protein 1

Alternate Names

basic-helix-loop-helix-PAS protein MOP2; bHLHe73; class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.