Rabbit anti-HINT1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-363A-T maps to a region between residue 1 to 50 of human Histidine triad nucleotide-binding protein 1 using the numbering given in entry P49773.2 (GeneID 3094).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HINT1 immobilized on solid support.
The epitope recognized by A305-363A maps to a region between residue 1 to 50 of human Histidine triad nucleotide-binding protein 1 using the numbering given in entry P49773.2 (GeneID 3094).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Histidine triad nucleotide-binding protein 1 (HINT1) hydrolyzes purine nucleotide phosphoramidates substrates, including AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif [taken from NCBI Entrez Gene (Gene ID: 3094)].
Alternate Names
adenosine 5'-monophosphoramidase; epididymis secretory sperm binding protein; HINT; histidine triad nucleotide-binding protein 1; NMAN; PKCI-1; PRKCNH1; protein kinase C inhibitor 1; protein kinase C-interacting protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.