Rabbit anti-HIP1R/HIP12 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
HIP1R is a component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. It binds 3-phosphoinositides (via ENTH domain). HIP1R may act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75146].
Alternate Names
HIP12; HIP-12; HIP1-related protein; HIP3; huntingtin interacting protein 12; Huntingtin-interacting protein 12; huntingtin-interacting protein 1-related protein; ILWEQ
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.