Rabbit anti-HJURP Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
Holliday junction recognition protein (HJURP) is a centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. HJURP acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. HJURP directly binds Holliday junctions [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8NCD3].
Alternate Names
14-3-3-associated AKT substrate; FAKTS; fetal liver expressing gene 1; fetal liver-expressing gene 1 protein; hFLEG1; Holliday junction recognition protein; up-regulated in lung cancer 9; URLC9
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.