Rabbit anti-hnRNP-L Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
hnRNP-L is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HnRNP-L Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. It associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter cytoplasm [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14866].
Alternate Names
epididymis secretory sperm binding protein; heterogeneous nuclear ribonucleoprotein L; hnRNP L; hnRNP-L; HNRPL; P/OKcl.14
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.