Rabbit anti-hSET1B Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-280A-T maps to a region between residue 350 and 400 of human SET domain containing 1B using the numbering given in entry NP_055863.1 (GeneID 23067).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to hSET1B immobilized on solid support.
The epitope recognized by A302-280A maps to a region between residue 350 and 400 of human SET domain containing 1B using the numbering given in entry NP_055863.1 (GeneID 23067).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
hSET1B is a histone methyltransferase that associates in a complex with the non-catalytic subunits CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. These five non-catalytic subunits are also part of the Set1A complex. The SET1B complex has been shown to catalyze the tri-methylation of ‘Lys-4’ on histone H3. SET1B has been shown to localize to sites independent of SET1A complexes indicating non-redundant functions between SET1B and SET1A complexes.
Alternate Names
histone-lysine N-methyltransferase SETD1B; hSET1B; KMT2G; lysine N-methyltransferase 2G; SET domain-containing protein 1B; Set1B
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.