Rabbit anti-HSPA9/GRP75/Mortalin Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-256A maps to a region between residue 629 to 679 of human Stress-70 protein, mitochondrial using the numbering given in entry P38646.2 (GeneID 3313).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to HSPA9/GRP75/Mortalin immobilized on solid support.
The epitope recognized by A305-256A-T maps to a region between residue 629 to 679 of human Stress-70 protein, mitochondrial using the numbering given in entry P38646.2 (GeneID 3313).
Additional Product Information
HSPA9 a member of the heat shock protein 70 gene family. HSPA9 is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria taken from NCBI Entrez Gene (Gene ID: 3313)].
Alternate Names
75 kDa glucose-regulated protein; catecholamine-regulated protein 40; CRP40; CSA; epididymis secretory sperm binding protein Li 124m; EVPLS; GRP75; GRP-75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); HEL-S-124m; HSPA9B; Mortalin; mortalin, perinuclear; mortalin2; mortalin-2; MOT; MOT2; MTHSP75; p66-mortalin; PBP74; peptide-binding protein 74; SAAN; SIDBA4; stress-70 protein, mitochondrial
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.