Rabbit anti-IFN-gamma Recombinant Monoclonal Antibody [BLR300L]
Product Details
Specifications
Additional Product Information
IFN-gamma is a soluble cytokine that is a member of the type II interferon class. It is produced by immune cells such as T-cells and NK cells and plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. [taken from NCBI Entrez Gene (Gene ID: 3458)].
Alternate Names
IFG;IFI;IFN-gamma;immune interferon;interferon gamma
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.