Rabbit anti-INT1 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
INT1 was first identified as KIAA1440, a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. Targeted disruption of INT1 results in growth arrest at the early blastocyst stage, activated caspase-3/7 that results in apoptosis, and impaired processing of U2 snRNA. INT1 has been demonstrated to be the largest subunit of the integrator complex which functions to mediate U1 and U2 snRNA 3’end processing.
Alternate Names
INT1; integrator complex subunit 1; NET28
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.