Rabbit anti-IPO9 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-475A maps to a region between residue 991 to 1041 of human Importin-9 using the numbering given in entry Q96P70.3 (GeneID 55705).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IPO9 immobilized on solid support.
The epitope recognized by A305-475A-T maps to a region between residue 991 to 1041 of human Importin-9 using the numbering given in entry Q96P70.3 (GeneID 55705).
Additional Product Information
Importin-9 (IPO9) functions in nuclear protein import as a nuclear transport receptor. IPO9 serves as receptor for nuclear localization signals (NLS) in cargo substrates. It is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). IPO9 mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. It prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. IPO9 may also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96P70].
Alternate Names
Imp9; importin-9; ran-binding protein 9; ranBP9
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.