Rabbit anti-IRS1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-157A maps to a region between residue 750 and 800 of human insulin receptor substrate 1 using the numbering given in entry NP_005535.1 (GeneID 3667).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IRS1 immobilized on solid support.
The epitope recognized by A301-157A-T maps to a region between residue 750 and 800 of human insulin receptor substrate 1 using the numbering given in entry NP_005535.1 (GeneID 3667).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
The insulin receptor substrate 1 (IRS1) is one of four members of the IRS family (IRS1-4) that mediates intracellular signaling downstream of the IGF1 and insulin receptors. Phosphorylation of IRS1 by activated receptors leads to binding of IRS to the SH2 domains of effector proteins such as PI3K, Grb2, nck, and SHP2. The downstream effects of insulin and IGF1 signaling via IRSs on the cell are pleiotropic and include various responses such as glucose transport, glycogen synthesis, protein synthesis, antilipolysis, and gluconeogenesis. Polymorphisms in IRS1 may contribute to the etiology of non-insulin-dependent diabetes mellitus (NIDDM).
Alternate Names
HIRS-1; insulin receptor substrate 1; IRS-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.