Rabbit anti-IRS4 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A305-344A maps to a region between residue 1207 to 1257 of human Insulin receptor substrate 4 using the numbering given in entry O14654.1 (GeneID 8471).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to IRS4 immobilized on solid support.
The epitope recognized by A305-344A-T maps to a region between residue 1207 to 1257 of human Insulin receptor substrate 4 using the numbering given in entry O14654.1 (GeneID 8471).
Additional Product Information
Insulin receptor substrate 4 (IRS4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation [taken from NCBI Entrez Gene (Gene ID: 8471)].
Alternate Names
160 kDa phosphotyrosine protein; insulin receptor substrate 4; IRS-4; phosphoprotein of 160 kDa; pp160; PY160
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.