Rabbit anti-JAM-A Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A302-891A maps to a region between residue 249 and 299 of human Junctional Adhesion Molecule A using the numbering given in entry NP_058642.1 (GeneID 50848).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to JAM-A immobilized on solid support.
The epitope recognized by A302-891A-T maps to a region between residue 249 and 299 of human Junctional Adhesion Molecule A using the numbering given in entry NP_058642.1 (GeneID 50848).
Additional Product Information
Junctional adhesion molecule A (JAM-A) is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor [taken from NCBI Entrez Gene (Gene ID: 50848)].
Alternate Names
CD antigen CD321; CD321; JAM; JAM1; JAM-1; JAMA; JAM-A; JCAM; junctional adhesion molecule 1; junctional adhesion molecule A; KAT; PAM-1; platelet adhesion molecule 1; platelet F11 receptor
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.