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Rabbit anti-JHDM1A Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
JHDM1A
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 825 and 875
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to JHDM1A immobilized on solid support.

The epitope recognized by A301-475A maps to a region between residue 825 and 875 of human JmjC domain-containing histone demethylation protein 1A using the numbering given in entry NP_036440.1 (GeneID 22992).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to JHDM1A immobilized on solid support.

The epitope recognized by A301-475A-T maps to a region between residue 825 and 875 of human JmjC domain-containing histone demethylation protein 1A using the numbering given in entry NP_036440.1 (GeneID 22992).
Country of Origin
USA

Additional Product Information

JHDM1A is a member of the JHDM1 histone demethylase family that contains JmjC domains and regulates transcription. It is also a member of the F-box family of proteins which are components of the SKP1-cullin-F-box (SCF) ubiquitin-protein ligase complex. In addition to the JmjC and F-box domains, JHDM1A also contains a CXXC-type zinc-finger, a PHD-type zinc-finger, and 3 LRR (leucine-rich) repeats.

22992
KDM2A
lysine demethylase 2A
Q9Y2K7
Lysine-specific demethylase 2A

Alternate Names

Histone-H3-lysine-36 demethylase 1A; CXXC8; CXXC-type zinc finger protein 8; FBL11; FBL7; F-box and leucine-rich repeat protein 11; F-box protein FBL7; F-box protein Lilina; F-box/LRR-repeat protein 11; FBXL11; JHDM1A; jmjC domain-containing histone demethylation protein 1A; jumonji C domain-containing histone demethylase 1A; LILINA; lysine (K)-specific demethylase 2A; lysine-specific demethylase 2A

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.