Rabbit anti-Junctophilin 1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-593A maps to a region between residue 450 to 500 of human Junctophilin 1 using the numbering given in entry NP_065698.1 (GeneID 56704).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Junctophilin 1 immobilized on solid support.
The epitope recognized by A304-593A-T maps to a region between residue 450 to 500 of human Junctophilin 1 using the numbering given in entry NP_065698.1 (GeneID 56704).
Additional Product Information
Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. Junctophlin 1 contributes to the construction of the skeletal muscle triad by linking the t-tubule (transverse-tubule) and SR (sarcoplasmic reticulum) membranes [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HDC5].
Alternate Names
CMT2K; JP1; JP-1; junctophilin type 1; junctophilin-1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.