Rabbit anti-Junctophilin-2 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A304-455A maps to a region between residue 625 to 675 of human Junctophilin-2 using the numbering given in entry NP_065166.2 (GeneID 57158).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Junctophilin-2 immobilized on solid support.
The epitope recognized by A304-455A-T maps to a region between residue 625 to 675 of human Junctophilin-2 using the numbering given in entry NP_065166.2 (GeneID 57158).
Additional Product Information
Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Junctophilins provide a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. Junctophilin-2 is necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release, and contributes to the construction of skeletal muscle triad junctions [Taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9BR39].
Alternate Names
CMH17; JP2; JP-2; junctophilin type 2; junctophilin-2
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.