Rabbit anti-KANK1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A301-882A maps to a region between residue 1302 and 1352 of human KN motif and ankyrin repeat domains 1 using the numbering given in entry NP_055973.2 (GeneID 23189).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to KANK1 immobilized on solid support.
The epitope recognized by A301-882A-T maps to a region between residue 1302 and 1352 of human KN motif and ankyrin repeat domains 1 using the numbering given in entry NP_055973.2 (GeneID 23189).
Additional Product Information
The gene that encodes KANK1 (KN motif and ANKyrin repeat-domain-containing protein 1) was originally identified in a chromosomal region that exhibited loss of heterozygosity in renal cell carcinoma. KANK1 bears five ankyrin repeats and a conserved motif at the N-terminus which contains motifs for nuclear localization and export signals (KN-motif). It functions to regulate actin stress fiber formation and has been found to be a substrate of Akt and to bind 14-3-3.
Alternate Names
ANKRD15; ankyrin repeat domain-containing protein 15; CPSQ2; KANK; kidney ankyrin repeat-containing protein; KN motif and ankyrin repeat domain-containing protein 1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.