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Rabbit anti-KIAA0082 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
KIAA0082
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 125 and 175
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to KIAA0082 immobilized on solid support.

The epitope recognized by A300-304A maps to a region between residues 100 and 150 of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to KIAA0082 immobilized on solid support.

The epitope recognized by A300-304A-T maps to a region between residues 100 and 150 of human KIAA0082 using the numbering given in TrEMBL entry Q8N1G2 (GeneID 23070).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

KIAA0082 was first identified as a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. KIAA0082 contains a G-patch and WW domain and several predicted phosphoserine sites.

23070
CMTR1
cap methyltransferase 1
Q8N1G2
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Alternate Names

Cap methyltransferase 1; cap1 2'O-ribose methyltransferase 1; cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1; FtsJ methyltransferase domain containing 2; ftsJ methyltransferase domain-containing protein 2; FTSJD2; hMTr1; interferon-stimulated gene 95 kDa protein; ISG95; KIAA0082; MTr1; S-adenosyl-L-methionine-dependent methyltransferase FTSJD2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.