Rabbit anti-KIAA1033/WASH complex subunit 7 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
KIAA1033/WASH complex subunit 7 acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes sems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in the regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q2M389].
Alternate Names
KIAA1033; MRT43; strumpellin and WASH-interacting protein; SWIP; WASH complex subunit 4; WASH complex subunit 7; WASH complex subunit SWIP
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.