Rabbit anti-KPNB1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-482A maps to a region between residue 825 and the C-terminus (residue 876) of human Karyopherin (importin) beta 1 using the numbering given in entry NP_002256.2 (GeneID 3837).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to KPNB1 immobilized on solid support.
The epitope recognized by A300-482A-T maps to a region between residue 825 and the C-terminus (residue 876) of human Karyopherin (importin) beta 1 using the numbering given in entry NP_002256.2 (GeneID 3837).
Additional Product Information
KPNB1, also known as importin (karyopherin)-beta, plays an important role in the transport of macromolecules between the cytoplasm and nucleus. KPNB1 functions as a transport receptor that binds to importin-alpha-bound cargo and docks it to the cytoplasmic side of the nuclear pore complex (NPC) for translocation through the NPC.
Alternate Names
IMB1; Impnb; importin 1; importin 90; importin beta-1 subunit; importin subunit beta-1; Importin-90; IPO1; IPOB; karyopherin (importin) beta 1; Karyopherin subunit beta-1; NTF97; nuclear factor p97; pore targeting complex 97 kDa subunit; PTAC97
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.