/ Antibodies & Antisera / Primary Antibodies / Rabbit anti-KT3 Tag Antibody
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Primary Antibodies  

Rabbit anti-KT3 Tag Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog # A190-129A
Target:
KT3 Tag
Applications:
ELISA, ICC, IP, WB
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
Unconjugated (0.1 mg)

A190-129A
$268.00
Qty:
Bulk Request

Product Details

Specifications

Concentration
1 mg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
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Production Procedures
Rabbits were immunized with KT3 (KPPTPPPEPET) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

The KT3 tag is an epitope tag with an 11-residue sequence of KPPTPPPEPET. Derived from the large T-antigen of SV40, KT3 tags can help with detection of proteins in western blot, IP, or microscopic techniques.

Applications

Not all listed applications have been specifically tested by our laboratory. All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.