Rabbit anti-LATS1 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-478A maps to a region between residue 1075 and the C-terminus (residue 1130) of human Large Tumor Suppressor homolog 1 using the numbering given in SwissProt entry O95835 (GeneID 9113).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to LATS1 (WARTS) immobilized on solid support.
The epitope recognized by A300-478A-T maps to a region between residue 1075 and the C-terminus (residue 1130) of human Large Tumor Suppressor homolog 1 using the numbering given in SwissProt entry O95835 (GeneID 9113).
Additional Product Information
LATS1 (Large Tumor Suppressor homolog 1) has been identified as a tumor suppressor protein linked to sarcomas and ovarian cancer. LATS1 is a member of the Dbf2-related (NDR) family of protein kinases that are key components of the mitotic exit network. LATS1 interacts with MOB1 and CDC2 kinase in early mitosis. LATS1 appears to negatively regulate G2/M transitioning by down-regulating CDC2 kinase activity.
Alternate Names
h-warts; large tumor suppressor homolog 1; LATS (large tumor suppressor, Drosophila) homolog 1; LATS, large tumor suppressor, homolog 1; serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.