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Rabbit anti-LDLR Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
LDLR
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 810 and C-terminus
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$131.00 $464.00
Qty:

Product Details

Specifications

Verified Reactivity
Human
Presumed Reactivity
Mouse, Rabbit, Bovine, Rat
Antigen Species
Human
Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Production Procedures
Antibody was affinity purified using an epitope specific to LDLR immobilized on solid support.

The epitope recognized by A304-417A-T maps to a region between residue 810 to 860 of human Low Density Lipoprotein Receptor using the numbering given in entry NP_000518.1 (GeneID 3949).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Antibody was affinity purified using an epitope specific to LDLR immobilized on solid support.

The epitope recognized by A304-417A maps to a region between residue 810 to 860 of human Low Density Lipoprotein Receptor using the numbering given in entry NP_000518.1 (GeneID 3949).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Country of Origin
USA

Additional Product Information

Low-density lipoprotein receptor, LDLR, binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, LDLR functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cell [taken from NCBI Entrez Gene (Gene ID: 3949)].

3949
LDLR
low density lipoprotein receptor
P01130
Low-density lipoprotein receptor

Alternate Names

FH; FHC; LDL receptor; LDLCQ2; low-density lipoprotein receptor; low-density lipoprotein receptor class A domain-containing protein 3

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.