Rabbit anti-LEDGF/p75 Antibody Affinity Purified
Product Details
Specifications
The epitope recognized by A300-847A-T maps to a region between residue 225 and 275 of human Lens Epithelium Derived Growth Factor/Transcriptional Coactivator p75 using the numbering given in entry NP_150091.2 (GeneID 11168).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to LEDGF/p75 immobilized on solid support.
The epitope recognized by A300-847A maps to a region between residue 225 and 275 of human Lens Epithelium Derived Growth Factor/Transcriptional Coactivator p75 using the numbering given in entry NP_150091.2 (GeneID 11168).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
LEDGF/p75 has been identified as a transcriptional coactivator involved in lens epithelial cell gene regulation and in neuroepithelial stem cell differentiation and neurogenesis. LEDGF has also been shown to function as a cofactor for lentivirus and HIV integration.
Alternate Names
CLL-associated antigen KW-7; dense fine speckles 70 kDa protein; DFS 70; DFS70; LEDGF; lens epithelium-derived growth factor; p52; p75; PAIP; PC4 and SFRS1-interacting protein; PSIP2; transcriptional coactivator p52/p75; Transcriptional coactivator p75/p52
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.