Rabbit anti-LIN54 Antibody Affinity Purified
Product Details
Specifications
Additional Product Information
LIN54 is a component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, LIN54 acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6MZP7].
Alternate Names
CXC domain-containing protein 1; CXCDC1; JC8.6; lin-54 homolog; MIP120; protein lin-54 homolog; TCX1
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.